PLANT TISSUE CULTURE (2 CREDITS)

Learning Outcomes:

On successful completion of this course, students will be able to: LO1 – explain fundamental principles and techniques of plant tissue culture, including aseptic methods and media preparation; LO2 – Identify factors affecting in vitro plant growth and optimize culture conditions for different plant species; LO3 – Understand various plant regeneration methods, such as organogenesis and embryogenesis; LO4 – apply the applications and limitations of plant tissue culture in research, conservation, and commercial production.

Topics:

1. Fundamental of Plant Embryogenesis ;
2. Fundamental of Seed, Germination, and Seedling ;
3. Fundamental of Plant Vegetative Growth and Development ;
4. Introduction to Plant Tissue Culture (PTC) ;
5. PTC Tools and Media Preparation ;
6. Micropropagation Techniques ;
7. Somaclonal Variation ;
8. Callus Culture ;
9. Zygotic Embryo Culture ;
10. Somatic Embryogenesis ;
11. Synthetic Seed Technology ;
12. Protoplast Culture ;
13. Bioreactor for Secondary Metabolite Production.