GENETIC ENGINEERING (2/2 CREDITS)

Learning Outcomes:

On successful completion of this course, students will be able to: LO1 – explain Explain the principles of genetic engineering, including the fundamentals of molecular biology and ethical considerations in the application of genetic engineering.; LO2 – apply Apply fundamental tools and methodologies for gene manipulation ; LO3 – solve Solve a case study on environmental management, health management, or bioresource improvement by developing a genetic engineering solution through the integration of theoretical knowledge and laboratory techniques.

Topics:

1. Introduction to genetic engineering: story of DNA ;
2. Good Laboratory Practices in Genetic Engineering;
3. Bioinformatics Approach for Genetic Engineering;
4. Genetic engineering in context: Ethics ;
5. Molecular biology tools ;
6. Bioinformatics Approach for Genetic Engineering;
7. Plasmid Isolation ;
8. Working with nucleic acid;
9. Host and vector;
10. Generating DNA Fragments;
11. Cloning strategy;
12. Cloning strategies;
13. Polymerase chain reaction;
14. Recombinant Analysis;
15. CRISPR/Cas9 Project ;
16. Selection, screening, and analysis of recombinant;
17. Genome editing;
18. Group Project ;
19. Innovation in biotechnology: genetic engineering for product development;
20. Genetic engineering in medical field ;
21. Transgenic plant and animals;
22. Group Project ;
23. Introduction to synthetic biology.