ENZYME BIOTECHNOLOGY (2 CREDITS)

Learning Outcomes:

On successful completion of this course, students will be able to: LO1 – outline the role, structure, classification, characteristic, mechanisms, and regulation of enzymes as the biocatalyst within the living system; LO2 – explain the definition of enzyme activity, enzyme kinetics, assay technique for enzyme kinetics, order reactions, Michaelis-Menten equation, allosteric enzyme, and enzyme inhibitors; LO3 – Outline the application of enzymes in clinical diagnosis and industrial purpose, and technique in enzyme isolation; LO4 – demonstrate the calculation of enzyme kinetics using enzyme assay data and the implication of enzyme activity parameters to the biological activity in living system; LO5 – demonstrate the biochemistry techniques to perform extraction of enzymes, fractionation of enzyme, purification of enzyme, enzyme engineering, and characterization of enzyme as biomolecules within the living system.

Topics:

1. Introduction to Enzymology;
2. Enzyme Classification and Basic Chemistry;
3. Enzyme Function and Cofactors;
4. Enzyme Dynamics and Mechanisms;
5. Enzyme Activity Parameter & Assay Techniques;
6. Enzyme Kinetics I: Basic and Parameters;
7. Enzyme Kinetics II: Special Cases of Reaction;
8. Allosteric Enzymes & Enzyme Inhibition;
9. Applications of Enzymes;
10. Enzyme Isolation and Purification;
11. Visualization & Size Estimation;
12. Enzyme Immobilisation;
13. Enzyme Engineering & Structural Determination Techniques.