FERMENTATION TECHNOLOGY (2/2 CREDITS)

Learning Outcomes:

On successful completion of this course, students will be able to: LO1 – explain the principles of fermentation technology and apply microbial growth kinetics to optimize fermentation processes ; LO2 – apply the formulation of media, contamination control strategies, and inoculum culture for fermentation process; LO3 – apply bioreactors system for batch, fed-batch, and continuous fermentation processes and adopt scale-up principles for industrial fermentation; LO4 – solve problems related to upstream and downstream processing in microbial and plant cell bioprocess technology.

Topics:

1. Session 1 – Introduction to Fermentation Technology;
2. Practicum 1 – S. cerevisiae Growth Study (Media Preparation and Seed Culture);
3. Introduction to fermentation technology and culture type ;
4. Fermentation media and Inoculum preparation ;
5. Practicum 2 – S. cerevisiae Growth Study (Initial Culture Characterization);
6. Session 2 – Fermentation and culture type ;
7. Session 3 – Fermentation media preparation ;
8. Practicum 3 – S. cerevisiae Growth Study (Shaking Incubator Operation and Culture Scale-up); Microbial Growth Kinetics and Modeling ;
9. Bioreactor Design, Upstream Processing, and Downstream Processing;
10. Practicum 4 – S. cerevisiae Growth Study (Growth Monitoring and Analysis);
11. Session 4 – Inoculum preparation ;
12. Session 5 – Microbial Growth Kinetics ;
13. Practicum 5 – S. cerevisiae Growth Study (Metabolite Analysis);
14. Fermentation Scale up Process and Computer system for fermentation;
15. Plant growth kinetics and Plant cell suspension culture in bioprocess ;
16. Practicum 6 – S. cerevisiae Growth Study (Data Integration and Reporting);
17. Session 6 – Microbial Growth Modeling;
18. Session 7 – Bioreactor Design ;
19. Practicum 7 – J. turmeric Growth Study (Aseptic Technique and Media Preparation);
20. Practicum 8 – J. turmeric Growth Study (Explant Preparation and Culture Initiation);
21. Session 8 – Upstream Processing ;
22. Session 9 – Downstream Processing ;
23. Practicum 9 – J. turmeric Growth Study (TIS-RITA Assembly and Culture inoculation);
24. Practicum 10 – J. turmeric Growth Study (Monitoring and Maintenance);
25. Session 10 – Fermentation Scale up Process ;
26. Session 11 – Computer system for fermentation ;
27. Practicum 11 – J. turmeric Growth Study (Monitoring and Maintenance);
28. Practicum 12 – J. turmeric Growth Study (Monitoring and Maintenance);
29. Session 12 – Plant growth kinetics ;
30. Session 13 – Plant cell suspension culture in bioprocess ;
31. Practicum 13 – J. turmeric Growth Study (Analysis and Documentation).