BIOCHEMISTRY (2/2 CREDITS)

Learning Outcomes:

On successful completion of this course, students will be able to: LO1 – explain the structure, classification, characteristic, and roles of biomolecules withing the living system; LO2 – outline the mechanisms and regulation of enzymes as the biocatalys in the metabolism of living system; LO3 – illustrate the metabolic pathways of biomolecules (carbohydrates, lipids, amino acids, and nucleotides) and their integration in living systems; LO4 – Explain the principles of bioenergetics and the energy-producing pathways including glycolysis, the citric acid cycle, oxidative phosphorylation, and photosynthesis; LO5 – demonstrate the biochemistry laboratory techniques to perform the biochemical assay, measure enzyme activity, and execute the separation of various biomolecules.

Topics:

1. Fundamental aspect of biochemistry;
2. General laboratory principles;
3. Basic techniques and procedures in biochemistry;
4. Amino acids, peptides, and protein;
5. Determination of Protein;
6. Enzymes;
7. Thin Layer Chromatography: pigments; Carbohydrate;
8. Bromelain: extraction and enzyme activity;
9. Bromelain: extraction and enzyme activity;
10. Lipid and membranes;
11. Determination of total carbohydrate;
12. Carbohydrate metabolism;
13. Estimation of reducing and non-reducing sugar;
14. Aerobic metabolism;
15. Lipid metabolism;
16. Determination of free fatty acids;
17. Nitrogen metabolism;
18. Saponification;
19. Integration of metabolism;
20. Estimation of antioxidant activity;
21. Simple DNA isolation;
22. Nucleic Acids.